We are dedicated to implementing capillary electrophoresis mass spectrometry for comprehensive and quantitative bottom-up and top-down proteomics of zebrafish embryos and single blastomeres in the embryos during early embryogenesis. Our research will provide new insights into many important events during early vertebrate embryogenesis, e.g., early cellular differentiation and zygotic genome activation, thus leading to better understanding of birth defects that usually originate during early embryogenesis.